Journal of Aquaculture Research & Development

Evaluation of germplasm resources and analysis of genetic diversities from the ridgetail white prawn Exopalaemon carinicauda

International Conference on Aquaculture & Fisheries

July 20-22, 2015 Brisbane, Australia

Jitao Li

Posters-Accepted Abstracts: J Aquac Res Development

Abstract:

The ridgetail, white prawn (Exopalaemon carinicauda) is one of themajor economic shrimp species, which naturally distributes in thecoasts of Yellow Sea and Bohai Sea, China. Due to the multiple merits of fast growth, good reproductive performance and good environmental adaptability, the culture area of E. carinicauda has expanded in recent years and contributes to one third of the gross outcome of the polyculture ponds in eastern China. The current cultural pattern depends on wild seedingsor parent prawns from the natural sea area, ambiguous genetic background and destabilizing supply heavily influenced the development for culture of E. carinicauda. However, few studies on germplasm resources and genetic diversities in E. carinicauda have been reported. In the present study, fivegeographic populations were collected from coastal waters of Bohai and the Yellow Sea since 2011. The regression equation for seven morphological traits to body weight was built using multivariate analysis, the results indicated body length had the most direct impact on weight with path analysis. The results of karyotype analysis showed 2n=56m+8sm+12st+14t=90. The results of isozyme analysis suggested phylogenetic relationship between populations of Bohai bay and Laizhou bay was close, and followed by populations of Jiaozhou bay and Haizhou bay. The genetic diversity of the population and phylogenetic relationship among Palaemonidae were analyzed based on mitochondrion DNA 16S rRNA, COI sequence and ribosome ITS1 sequence, the results indicated the genetic differentiation coefficient between populations of Xiangshan bay and Laizhou bay was highest, and that was lowest between populations of Laizhou bay and Haizhou bay. A microsatellite-enriched library was constructed using the method of magnetic bead hybridization enrichment and fifty-six polymorphic microsatellite primers were screened, and the genetic diversity of populations was analyzed using twelve polymorphic microsatellite primers, UPGMA clustering tree based onDgenetic distance showed that Laizhou Bay stock was more closely related to Xiangshan stock and Haizhou Bay stock was farthest related to Xiangshang stock. In summary, the above results provided basic data for the protection and utilization for germplasm resources of E. carinicauda.