Journal of Bioequivalence & Bioavailability

Estimation of intestinal permeability of E and Z guggulsterone using Mandin Darby Canine Kidney (MDCK) cell line

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

Rajbir Singh, Jagadeesh Panduri, Y.S Chhonker, Devendra Kumar, Deepak Kumar, Pankaj Shrivastava, Vijay Kumar and R.S. Bhatta

Posters: J Bioequiv Availab

Abstract:

T he gum resin of the tree Commiphora mukul has been used in Ayurvedic medicine to treat a variety of ailments, including obesity, arthritis, inflammation, and lipid disorders. The active compounds in this resin are the cis and trans isomers of guggulsterone (GS) (4,17(20)-pregnadiene-3,16-dione). Intestinal absorption potential of E & Z isomers across the Mandin Darby Canine Kidney (MDCK) was calculated using HPLC-UV method. Method was validated in terms of accuracy, precision, selectivity, sensitivity, recovery. Apparent permeability coefficient of E isomer from apical to basal and basal to apical was 9.24 ? 0.013?10 -5 cm/sec and 1.07 ? 0.08?10 -4 cm/sec respectively. Similarly apparent permeability coefficient of Z isomer from apical to basal and basal to apical was 7.84 ? 0.093 ?10 -5 cm/sec and 8.45 ? 0.3 ?10 -5 cm/sec. Lucifer yellow and Rhodamine 123 were used as paracellular and P-gp marker substrate and apparent permeability coefficient was measured