Journal of Bioequivalence & Bioavailability

Estimation of biogenic amines concentration from Cucurbita Pepo treated rat brains: Implications for antiamnesic potential

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

Rahul Shivaji Adnaik

Posters: J Bioequiv Availab

Abstract:

T raditional Indian literature reports the role of Cucurbita pepo in various nervous disorders. The study was aimed to investigate the effect of n-hexane extract of C. pepo (HECP) on biogenic amines ? noradrenaline (NA), dopamine (DA), serotonin (5- HT) and Gamma amino butyric acid (GABA) concentrations in rat brain after induction of amnesia with scopolamine. Animals were treated with the HECP (100 mg/kg and 200 mg/kg) and piracetam (100 mg/kg). On 14 th day, animals were killed by cervical dislocation and brains were dissected out. Brains were homogenized with dry n-butanol and then centrifuged. About 4 ml aliquots of the clear supernatant were extracted with 3 ml of 0.1M phosphate buffer. Then, after adding 4% EDTA, 0.2 ml iodine solution, 0.5 ml alkaline sulphite and 0.6 ml 5N acetic acid, the solutions were heated and cooled. The intensities of fluorescence in resulting solutions were determined flurometrically at wavelengths of 400/500 & 310/365 for epinephrine, norepinephrine and dopamine respectively. The concentration of 5 HT in the solution was calculated from the standard curves. Paper chromatographic method was adopted for GABA, glutamate and glutamine analysis. The results showed that HECP significantly increased NA level by 45%, 5-HT level by 44% as compared to respective control group. The extract also significantly reduced the DA and GABA levels by 30 and 49%, respectively as compared to respective control group. Thus, this study suggests t hat HECP extract has a definite role in restoring the monoamines in rat brain, which might be useful in treatment of amnesia