Health Care : Current Reviews

Establishment of Bacillus thuringiensis based exogenous double-stranded RNA production platform

Joint Event on International Conference on Applied Microbiology and Microbial Biotechnology & International Conference on Microbiome R&D and Biostimulants & 3^rd International Conference on Internal Medicine & Hospital Medicine

October 15-16, 2018 Ottawa, Canada

Min Gu Park

Seoul National University, Republic of Korea

Posters & Accepted Abstracts: Health Care Current Reviews

Abstract:

RNA interference (RNAi) has been considered as an alternative strategy to control agricultural pests whereby double-stranded RNA (dsRNA) triggers a potent and specific inhibition of its homologous mRNA. Since small dsRNAs are required for various RNAi applications, there is a need for cost-effective methods for producing large quantities of high-quality dsRNA. To produce exogenous dsRNA through simple and cost-effective methods, Bacillus thuringiensis (Bt) based dsRNA production platform was established. For this, Bt shuttle vector, pHT1K-vp1, which transcribes sense and the anti-sense VP1 gene of Sacbrood virus (SBV) under the control of sporulation-dependent cyt1Aa promoter with STAB-SD sequence was constructed and transformed into Bt 4Q7 strain. Transcription of the VP1 gene was analyzed using qPCR and Northern blot analysis. In addition, the dsRNA against VP1 gene produced from the Bt successfully suppressed the replication of SBV. These results suggested that the Bt potentially exploited as a new platform for dsRNA production.

Biography :

E-mail: redmango42@gmail.com