Journal of Stem Cell Research & Therapy

Establishment of αMHC-EGFP knock-in reporter human pluripotent stem cell line (SNUe003-A-3) by CRISPR/ Cas9-based genome editing

Joint Event on International Conference on STEM CELL AND ORTHOPEDICS & International Conference on PSYCHOSOMATIC AND LABORATORY MEDICINE

July 19-20, 2023 | Rome, Italy

Ha Myoung Lee

Catholic Kwandong University, Republic of Korea

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

The cardiac muscle-specific protein, α-myosin heavy chain (αMHC), is a major component of cardiac muscle filaments involved in cardiac muscle contraction. Here, we established an αMHC-enhanced fluorescent protein (EGFP) knock-in human pluripotent stem cell (hPSC) line by linking the EGFP gene to the C-terminal region of αMHC via a 2A non-joining peptide using CRISPR/Cas9 nuclease. The EGFP reporter precisely reflected the endogenous level of αMHC upon the induction of cardiac differentiation. This reporter cell line will be a valuable platform for cardiotoxicity tests, drug screening, and investigating the pathological mechanisms of cardiomyocytes.

Biography :

Hamyoung Lee completed a master's degree at Catholic Kwandong University in republic of korea. She conducted a study to establish a knock-in cell line using hPSC, and published it in the journal Stem Cell Research. Since then, research using hPSC has been conducted at Seoul National University's Institute of Reproductive Medicine and Population, Medical Research Center, and is mainly related to Adrenoleukodystrophy (ALD), a disease caused by the accumulation of Very long-chain fatty acids (VLCFAs) in the adrenal cortex and white matter by mutations in the ABCD1 gene located in the X chromosome (X q28).