Health Care : Current Reviews

Erythrocyte micro-vesciculations in health and diseases

8^th Global Conference on Nursing and Healthcare & 5^th Annual Conference on Diabetes and Endocrinology

JUNE 28, 2022 | WEBINAR

Ugochukwu O Maluze

Torrens University, Australia

Scientific Tracks Abstracts: Health Care Curr Rev

Abstract:

Heterogeneous mixture of vesicular organelle-like structures (micro-vesicles) is emitted by many cell sources into their surroundings, including blood and body fluids. Micro-vesicles are small membrane-enclosed sacs, whose size varies from 0.1-1nm; and they are thought to be shed from a variety of cell types, constitutively when stimulated by calcium activation signals. They seem to enhance the potential information transfer between cell types, displaying a large number of proteins and lipids as membrane constituents and as components of the vesicular content. Their isolation and analysis from blood samples have the potential to provide information about state and progression of malignancy and should prove of great clinical importance as biomarkers for a variety of disease states. They are implicated in normal, physiological and pathological conditions and their potential to serve as indicators in the diagnosis, prognosis and surveillance of a variety of health conditions, have indicated the magnitude of interest in these structures. In this Laboratory research report, non-induced microvesicles were isolated from erythrocytes and analysed using Guava Easy flow- cytometer. Considering the fact that microvesicles compartmentalisation consist of proteins, lipid and nucleic acid, the protein concentration of micro-vesicles was quantify and estimated using biuret method of assay; and the protein components were separated using agarose gel electrophoresis. The result of this present report was in consistency with the previous research work done by several authors, which shows that non- induced micro-vesicles are shed from erythrocytes; and secondary, that micro-vesicles skeletal structure consist of protein, lipid and nucleic acid.

Biography :

Ugochukwu O Maluze is an ambitious, dedicated, diligent, self-motivated and hardworking young man with an excellent achievements. He has completed his BSc. degree on Medical Laboratory Science from Ebonyi State University Nigeria. He has also completed his postgraduate qualifications on MSc. degrees on Biomedical Science (London Metropolitan University); MSc. Biomedical Engineering (University of Bedfordshire, UK); MSc. Public Health Advanced (Torrens University Australia) and a course on Clinical toxicology (London Metropolitan University, UK).