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Efficient inhibition of methicillin resistant-vancomycin intermediate Staphylococcus aureus (MRSA-VISA) by soybean glycinin basic subunit
International Conference on Food Safety and Regulatory Measures
August 17-19, 2015 Birmingham, UK
Gamal Enan, Gamal El-Didamony, Mahmoud Sitohy, Marwa Khalifa and Ali Osman
Zagazig University, Egypt
Scientific Tracks Abstracts: J Food Process Technol
Abstract:
One hundred and fifty Staphylococcus aureus (S. aureus) isolates were obtained from clinical samples characterized and identified. Out of this collection, thirty isolates were found to be methicillin resistant Staphylococcus aureus (MRSA). Only one strain (isolate No.P59) out of this subgroup exhibited reduced susceptibility to vancomycin, recoding 8 and 10 μg/mLMIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) respectively. This strain was concluded as vancomycin intermediate S. aureus (VISA) and designated VISA P59. This VISA P59 further identified by 16S rRNA sequence analysis and PCR analysis revealed that it contained methicillin resistant gene (mec A) in its genome but did not contain vancomycin resistant genes (van A and van B).The basic subunits of glycinin isolated from soybean protein (GBS) inhibited vigorously VISA P59 as qualitatively visualized by agar well diffusion method. GBS antimicrobial activity against VISA was stable over a wide pH range and under different incubation temperatures. It could prevent the in vitro growth of VISA P59 at 37ºC for 4 days and inhibited almost 99% of viable growth of VISA P59 in minced beef meat stored at 37ºC for 14 days. No animal toxicity was observed with GBS.
Biography :
Gamal Enan has completed his Ph.D. in Microbiology. He is a Professor and Chairman of Research Group of Bacteriology, Faculty of Science, and Zagazig University, Egypt (2009- up till now) .He is member in European Society of Microbiology, Immunology and Infectious diseases, American Society of Industrial Microbiology. He supervised 10 Ph.D. and 15 M.Sc. Degrees. He is a Supervisor of anther 20 thesis.
Email: gamalenan@ymail.com