Agrotechnology

Effect of plant growth regulators on in vitro regeneration from mature wheat (Triticum aestivum L.) embryo explants

13^th International Conference on Agriculture & Horticulture

September 10-12, 2018 | Zurich, Switzerland

Doriana Bode Xhulaj

Agricultural University of Tirana, Albania

Posters & Accepted Abstracts: Agrotechnology

Abstract:

The experiment was carried in the laboratory of plant tissue cultures, of the Institute on Plant Genetic Resources, Agriculture University of Tirana during 2016. An efficient method was developed for multiple shoot regeneration of wheat from mature embryos without callus formation, for a short period of time (2.5 months). Direct shoot regeneration is important since fewer somaclonal variations are likely to arise in indirect regeneration method. The effect of explants, cut shoot embryonic meristem and shoot embryonic meristem with scutellum and various combinations of plant growth regulators in MS media on direct shoot regeneration of wheat was investigated. Among different combination of 2,4-D, IBA, NAA and BAP tested, embryo explants cultured in MS medium supplemented with 2 mg/L BAP, 0.6 mg/L 2,4- D resulted in the most efficient direct shoot regeneration and produces a max of 2.5 shoot per explants and 3.4 cm shoot length. Plantlets were successfully transferred to rooting medium. The greatest mean numbers of roots were obtained on MS media supplemented with 0.5 mg/L NAA, and the best mean value of root length (8.2cm) at 0.5 mg/L IBA. In this paper by simply manipulating the concentrations of BAP, 2,4-D, IBA and NAA in the culture medium, is described a method rapidly obtaining whole plants without the subculture of wheat using embryo explants. Recent Publications: 1. Hamid K and Sadaf K (2014) Study of plant tissue culture technology: J. Biol. Chem. Research 31(2):1236-1244. 2. Mahmood I et al. (2012) Evaluation of tissue culture responses of promising wheat (Triticum aestivum L.) cultivars and development of efficient regeneration system: Pak. J. Bot. 44(1):277-284. 3. Raziuddin J et al. (2010) Effect of cultivars and culture medium on callus formation and plant regeneration from mature embryos of wheat (Triticum aestivum L.). Pak. J. Bot. 42(1):639-652. 4. Schulze J (2007) Improvements in cereal tissue culture by thidiazuron: a review. Fruit, Vegetable and Cereal Science and Biotechnology. 1(2):64-79. 5. Yu Y J et al. (2008) Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in China: Plant Breed. 127(3):249-255.

Biography :

Doriana Bode Xhulaj pursued her Doctorate Degree in Plant Biology Conservation from the Faculty of Natural Science of Tirana, Albania. She is a Researcher at the Institute of Plant Genetic Resources, part of Agricultural University of Tirana, Albania. She is the author of several papers in this field. She is currently involved in different project on plant breeding program.

E-mail: d.xhulaj@yahoo.com