Pharmaceutica Analytica Acta

Dual targeted peptide and folic acid decorated nanoparticles for the delivery of anti-cancer drug to glioma cell

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Udita Agrawal, S.P. Vyas

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Effective chemotherapy for glioblastoma requires a smart nanocarrier that can penetrate the blood brain barrier (BBB) and subsequently target the glioma cells. Dual-targeting paclitaxel (Ptx) polymeric nanoparticles (NPs) were produced by conjugating with both folate (FA) and cRGDfK, for the effective penetrating across BBB and targeting glioma, respectively. FA and cRGDfK modified NPs containing paclitaxel was prepared by nanoprecipitation technique. In vitro and in vivo studies demonstrated that the dual-targeting NPs could transport across the BBB and mainly distributed in the brain glioma. FA and cRGDfk conjugated NPs had maximum entrapment efficiency 81.34?3.41% (n=3). AFM showed NPs to be spherical and nanometric in size. The in vitro release followed in a biphasic pattern: i.e. an initial burst release followed by slower and sustained release and resulted in least haemolytic toxicity. The anti-tumor effect of the dual-targeting NPs also demonstrated increased survival time, decreased tumor volume and no significant effect on body weight. The dual-targeted NPs could improve the therapeutic efficacy of brain glioma and were found less toxic than the Ptx solution, showing a dual-targeting effect. These results indicate that dual-targeting NPs is a promising potential carrier for glioma chemotherapy.