Journal of Bioequivalence & Bioavailability

DNA-binding and Photocleavage Studies of Polypyridyl Cobalt(ІІІ) and Ruthenium(II) Mixed Ligand Complexes their ?light switch? on off effect

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

Nazar Mohammed Gabra, Bakheit Mustafa and S. Satyanarayana

Posters: J Bioequiv Availab

Abstract:

F our novel cobalt(ІІІ) and ruthenium(ІІ) complexes [Ru(bpy)2icpip] (bpy 2,2-bipyridine 1), [Ru(phen) 2 icpip] (phen 1,10-phenantroline; 2),[ Co(phen) 2 icpp] and [Co(bpy) 2 icpip], (icpip 2-(1H-Indol-3-yl)-1H-1,3,7,8-tetraaza-cyclopenta{L} phenanthrene are described. The complexes were characterized by elemental analysis, UV/VIS, IR , 1 H-NMR, 13 C-NMR and mass spectra. The binding of the complexes with calf thymus DNA has been investigated by absorption, emission spectroscopy, viscosity measurements, DNA melting, and DNA photocleavage. Experimental results indicate that the four complexes can interact in to DNA base pairs. Upon irradiation at 365nm, this complexes were found to promote the cleavage of plasmid pBR322 DNA from super coiled form І to nicked form ІІ , and the mechanism for DNA cleavage by complexes were also investigated