Oral Health and Dental Management

Determination of Staphylococcus species of dental plaque biofilms and their antibiotic susceptibility

18^th Asia-Pacific Dental and Oral Care Congress

November 21-23, 2016 Melbourne, Australia

Merih Kivanc, Alp Kayahan Kivanc, Burcin Guler and Zuhal Kirzioglu

Yeditepe University, Turkey
Anadolu University, Turkey
Süleyman Demirel University, Turkey

Posters & Accepted Abstracts: Oral Health Dent Manag

Abstract:

Dental plaque is the biofilm found naturally on teeth and it is the etiologic agent in dental caries and periodontal diseases. Staphylococcus spp. are not usually isolated from the oral cavity, and when this occurs, they are considered to belong to the transitory microbiota. Individuals with periodontal disease represent possible reservoirs of these opportunistic bacteria in the oral cavity. Patients with periodontal disease represent possible reservoirs of these opportunistic bacteria in the oral cavity. The aim of this study was to isolate and identify Staphylococcus spp. from dental plaque biofilm on the external surface of the teeth and determination of Staphylococcus spp. antibiotic susceptibility. After culture of the biological sample in brain heart infusion broth, Staphylococcus strains were isolated on blood agar plates (bioMe´rieux, Marcy lâ??Etoile, France) at 37°C in aerobic conditions for 24 h. Antibiotic resistance patterns of strains were determined by using agar disk diffusion method. Molecular characterization of isolated Staphylococcus spp. from dental plaque biofilm was performed using sequencing analysis of the 16S rRNA gene. Sequences were compared with NCBI GenBank entries using the BLAST algorithm (http://www.ncbi. nlm.nih.gov/BLAST). A total of 19 staphylococcal biofilm isolates comprising of various species from dental plaque samples were identified. S. pasteuri and S. epidermidis were found to be the most prevalent species. Analyzed isolates were resistant to 2 or 3 antimicrobials; a multi-drug resistant and methicillin-resistant isolate of Staphylococcus spp. was obtained.

Biography :

Email: mkivanc@anadolu.edu.tr