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Determination of gossypol and Cu-pyropheophytin A in edible oils by liquid chromatography tandem mass spectrometry
International Conference and Exhibition on Advances in HPLC & Chromatography Techniques
March 14-15, 2016 London, UK
Maw-Rong Lee and Pin-Yi Lin
National Chung Hsing University, Republic of China
Posters & Accepted Abstracts: Pharm Anal Acta
Abstract:
In 2013, the health harmful gossypol and Cu-pyropheophytin A were found in the edible oil in Taiwan, therefore, the safety of the edible oil was became an important issue in food safety. In this study, a simple, rapid and sensitive analytical method was developed for simultaneously determination of the gossypol and Cu-pyropheophytin A in edible oils by using dispersive solid-phase extraction (DSPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Gossypol and Cu-pyropheophytin A extracted from edible oils by using 5mL petroleum ether then adsorbed by 20 mg of chlorofilter sorbent could obtain the highest extraction efficiency. After desorbing by 1 mL of acetone, the extractant was analyzed by LC-MS/MS with atmospheric pressure chemical ionization (APCI). The proposed method was also applied to analyze edible oils purchased from the markets.
Biography :
Email: mrlee@dragon.nchu.edu.tw