Journal of Nanomedicine & Nanotechnology

Detection of genotoxicity of green synthesized silver, gold, and silver/gold bimetallic in human blood lymphocytes using comet assay

26^th International Conference on ADVANCED NANOSCIENCE AND NANOTECHNOLOGY

December 05-06, 2022 | Dubai, UAE

Naila Sher, Mushtaq Ahmed, Nadia Mushtaq and Rahmat Ali Khan

University of Science and Technology, Pakistan

Scientific Tracks Abstracts: J Nanomed Nanotechnol

Abstract:

In the present study, the possible genotoxic effects of silver, gold, and silver/gold bimetallic NPs (Ag, Au, and Ag/Au BNPs) in human blood lymphocytes were studied here by comet assay. Here, Ag, Au, and their Ag/Au BNPs were synthesized by using Hippeastrum hybridum (HH) extract. These NPs were studied by spectroscopic (UV-visible spectroscopy, FTIR, XRD, and EDX, and SEM analysis. XRD analysis confers the crystal structure with an average size of 13.3, 10.72, and 8.34 nm of Ag, Au, and Ag/Au BNPs respectively. SEM showed that Ag, Au, and Ag/Au BNPs had spherical morphologies, with nano measures calculated sizes of 40, 30, and 20nm respectively. EDX spectrometers confirmed the presence of elemental Ag signal of the AgNPs with 22.75%, Au signal of the AuNPs with 48.08%, Ag signal with 12%, and Au signal with 38.26% of the Ag/Au BNPs. The comet assay revealed that H2O2 and BNPs prompt high rates of DNA damage compared to plant, Ag, Au NPs, and non-treated lymphocytes, which are represented as the Total comet score (TCS). It is demonstrated that NPs interact with blood lymphocytes to cause significant DNA damage in a concentration-dependent manner. However, to reduce the potential threats of NPs further studies are recommended Keywords; Nanoparticle; Human lymphocyte; Comet; DNA damage Recent Publications 1. Naila Sher, Mushtaq Ahmed, Nadia Mushtaq, Rahmat Ali Khan. Enhancing antioxidant, antidiabetic, and antialzheimer performance of Hippeastrum hybridum (L.) using silver nanoparticles. Applied organometallic chemistry. (2022); 36: 6724. 2. Muhammad Amir Masooda, Yanping Wua, Yuncong Chena, Hao Yuana, Naila Sher, Faisal Faizd, Shankun Yaoa, Fen Qi, M. Irfan Khan, Mushtaq Ahmed, Nadia Mushtaq, Weijiang Hea, ZijianGuoa. Optimizing the photodynamic therapeutic effect of BODIPY-based photosensitizers against cancer and bacterial cells. Dyes and Pigments. (2022); 202: 110255. 3. Mushtaq Ahmed, Shan Zeb Khan, Naila Sher, Zia-Ur-Rehman, Nadia Mushtaq, Rahmat Ali Khan. Kinetic and toxicological effects of synthesized palladium(II) complex on snake venom (Bangarus sindanus) acetylcholinesterase. Journal of venomous animals and toxins including tropical diseases. (2021); 27: 09.

Biography :

Naila Sher is a PhD scholar in the Department of Biotechnology, Faculty of Biological Sciences, UST-Bannu, Pakistan. She is an visiting lecturer in University of Science and Technology, Pakistan. Her research interests are Nanomaterials, Nanomaterials Synthesis, Nanoparticle Preparation, XRD Analysis, X-ray Diffraction, SEM Analysis, Electrophoresis, Anticancer activity, Nanoparticles characterization, Nanoparticles characterization, Oxidative Stress Assays and Animal models. She has attended various workshops /Seminars and participated in many National and International Conferences.