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Detection of aflatoxigenic fungi in poultry feed by PCR
4th International Conference on Clinical Microbiology and Microbial Genomics
October 05-07, 2015 Philadelphia, USA
Raed Najeeb Al-Khersan
Basrah University, Iraq
Scientific Tracks Abstracts: Clin Microbiol
Abstract:
Aflatoxins are toxic secondary metabolites produced by Aflatoxigenic fungi: Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. These aflatoxins cause many diseases to human, animal and plants. Regulatory gen aflR regulates aflatoxin biosynthesis pathway. One hundred of pelted poultry feed samples from different broilers and broiler breedersā?? farm and from poultry feed stores and markets were collected. Fungi were isolated by culturing on PDA agar and MEA agar and sub-cultured on SDA agar. In total, 50 Aspergillus flavus strains were isolated and 34 of them were positively characterized as aflatoxigenic strains with size of 798 bp for aflR primers.
Biography :
Email: raednk@hotmail.com