Journal of Vaccines & Vaccination

Design and preparation of RNA vaccine against hepatitis C

5^th Asia Pacific Global Summit and Expo on Vaccines & Vaccination

July 27-29, 2015 Brisbane, Australia

Zarin Sharifnia

Posters-Accepted Abstracts: J Vaccines Vaccin

Abstract:

Hepatitis C virus is a blood borne disease estimated to infect more than 350 million people globally and is a leading cause of liver cirrhosis, transplantation and hepatocellular carcinoma. Current gold-standard therapy often fails, has significant side effects in many cases and is expensive. The fact that a significant proportion of infected people spontaneously control HCV infection in the setting of an appropriate immune response suggests that a vaccine for HCV is a realistic goal but no vaccine is currently available. The present study was designed to investigate the possibility of a new vaccine against the hepatitis C virus based on mRNA encoding of membrane antigens (Core &E2) of hepatitis C virus.Nucleotide sequence of mRNA encoding core and E2 antigen of HCV virus by bioinformatics program was design and in pGE plasmid vector was prepared. Then in vitro transcription reactions are used to synthesize mRNA from this recombinant DNA template. Nanoparticles encapsulated this mRNA synthesized and delivered to Monocytes isolated from human buffy coat and the activation and differentiation of monocyte to dendritic cells was examined. Immune response such as secretion of cytokines to HCV antigen can also be studied since cells grown on permeable supports with co-culture dendritic cells and T-cells of the same person.This project will be ongoing and final results of this study will be reported at the conference.