Journal of Bacteriology & Parasitology

Design and construction of 1,2,4-butanetriol-producing pathway in Escherichia coli

7^th World Congress on Microbiology

November 28-29, 2016 Valencia, Spain

Yanping Zhang, Fan Yang, Lei Sun, Xinghua Li, Taicheng Zhu and Yin Li

Chinese Academy of Sciences, China

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

1,2,4-butanetriol (BT) is an important non-natural chemical with a variety of industrial applications. Here we constructed a prototype strain for BT production from xylose by assembling a four-step synthetic pathway and disrupting the competing pathways in E. coli. By fine-tuning of the pathway enzymes expression level, the potential bottlenecks was identified and the BT production was increased by 4.3 fold, achieved a final titer of 1.58 g/L after 72 hours. Furthermore, we designed a novel six-step biosynthetic pathway for BT from malate for the purpose of using glucose as a cheap substrate. Following tests of several combinations of enzymes for the pathway, a five-enzymes-catalyzing-six-step pathway was constructed in E. coli. By assembling these enzymes, BT was detected in the fermentation broth upon addition of malate, proving BT can be biosynthesized from malate. As well, BT was detected in the fermentation using glucose as the sole carbon source, suggesting that such novel BT biosynthetic pathway has created the possibility for the production of BT from the cheaper substrate glucose.

Biography :

Email: zhangyp@im.ac.cn