Journal of Bioequivalence & Bioavailability

Derivative spectrophotometric methods for the determination of Zolpidem Tartrate

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

U. Sravani, B. Sai Pavan Kumar, P. Sowjanya and M. Mathrusri Annapurna

Posters: J Bioequiv Availab

Abstract:

A simple, rapid and sensitive difference spectrophotometric method was developed for the determination of Zolpidem tartrate in pharmaceutical dosage forms. Zolpi dem tartrate is a non benzodiazepine hypnotic agent binds preferentially to one benzodiazepine receptor subtype ω-1 bezodiazepine-1 thought to mediate hypnotic effects. The hypnotic actions of Zolpidem, like benzodiazepine hypnotics, are mediated at the benzodiazepine recognition site of the GABA receptor complex. Zolpidem behaves as a sleep inducer without the muscle relaxant and anticonvulsant effects of the benzodiazepines. A double beam UV- VIS spectrophotometer (UV-1800, Shimadzu) connected to computer loaded with spectra manager software UV Probe was employed with spectral bandwidth of 1nm and wavelength accuracy of ? 0.3 nm with a pair of 10 mm matched quartz cells. In this method, borate buffer solution was scanned by using phosphate buffer solution as blank. The drug solutions prepared in phosphate buffer were kept in place of reference cuvette and the difference in absorbance of the corresponding drug solutions prepared in borate buffer was recorded. The difference absorption spectrum shows maxima at 250.71 and minima at 291.31. The amplitude was recorded and a graph was plotted by taking the concentration of the solutions on the x-axis and the corresponding amplitude values on the y-axis. Zolpidem tartrate follow s Beer-Lambert?s law over the concentration range of 1.0-20 μg/ml (r 2 = 0.999). The % RSD in precision and accuracy studies was found to be less than 2.0. The proposed method was validated and can be successfully applied for the determination of Zolpidem tartrate in pharmaceutical formulations