Journal of Stem Cell Research & Therapy

Coupling osteogenesis and vasculogenesis for regeneration of skeletal tissues

7^th Annual Conference on Stem Cell and Regenerative Medicine

August 04-05, 2016 Manchester, UK

Esmaiel Jabbari

University of South Carolina, USA

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

Reconstruction of large bone defects is limited by insufficient vascularization and slow bone regeneration. The objective of this work was to investigate the effect of spatial and temporal release of recombinant human bone morphogenetic protein-2 (BMP2) and vascular endothelial growth factor (VEGF) on the extent of osteogenic and vasculogenic differentiation of human mesenchymal stem cells (hMSCs) and endothelial colony-forming cells (ECFCs) encapsulated in a patterned hydrogel. Nanogels (NGs) were used for grafting and timed-release of BMP2 and VEGF. hMSCs and NG-BMP2 were encapsulated in a patterned polyethylene glycol (PEG) based hydrogel matrix with microchannel patterns filled with a suspension of hMSCs+ECFCs and NG-VEGF in a gelatin hydrogel. Groups included patterned constructs without BMP2/VEGF (None), with directly added BMP2/ VEGF and NG-BMP2/NG-VEGF. Based on the results, timed-release of VEGF in the microchannels in 10 days from NG(10) and BMP2 in the matrix in 21 days from NG(21) resulted in highest extent of osteogenic and vasculogenic differentiation of the encapsulated hMSCs and ECFCs compared to direct addition of VEGF and BMP2. Further, timed-release of VEGF from NG(10) in hMSC+ECFC encapsulating microchannels and BMP2 from NG(21) in hMSC encapsulating matrix sharply increased the expression ofbasic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β) in the patterned constructs. The results suggest that mineralization and vascularization are coupled by localized secretion of paracrine signaling factors by the differentiating hMSCs and ECFCs.

Biography :

Email: JABBARI@cec.sc.edu