Fermentation Technology

Continous production of lipopeptide biosurfactant pseudofactin in a bioreactor with foam overflow and cell recirculation

World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry

August 25-27, 2015 Valencia, Spain

Piotr Biniarz and Marcin Lukaszewicz

University of Wroclaw, Poland

Posters-Accepted Abstracts: Ferment Technol

Abstract:

Pseudofactin (PF) is a novel lipopeptide biosurfactant (surface active compound) that have been isolated from cultures of arctic bacteria Pseudomonas fluorescens BD5. PF exhibits good emulsification activity in comparison to synthethic detergents as well as antimicrobial, antifungal and antiadhesive activity. It can also act as an antitumor agent. Therefore, there are many potential applications for PF in medicine, industry, etc. To decrease costs of PF production we tested the possibility of culturing P. fluorescens BD5 in fed-batch and continous modes in 2.4-L bioreactor. The production of PF increased approximately 10-fold in comparison to Erlenmayer flasks cultures. Simultaneously we observed very high rate of foam production and high affinity of PF to foam. Therefore we tested foam overflow as a method for initial isolation and concentration of PF. The problem with microbial cell washing out was solved by the recirculation of bacterial cells that have migrated from the bioreactor vessel with foam. Here we present method for the continous production of PF in bioreactor. During cultivation, PF is isolated and partially purified thanks to foam overflow. Simultaneous optimization of cultivation medium and process parameters allowed us to produce PF in concentrations up to 800 mg/L in broth and even more concentrated in foam.

Biography :

Piotr Biniarz is a PhD student in biotechnology at University of Wroclaw, Poland. He has completed his MA from University of Wroclaw and held traineeships at University of Ulster, UK and University Lille1 (Lille Technical University), France. His PhD project concerns optimization of lipopeptide biosurfactants production through media and process parameters optimization, investigating innovative methods for purification of lipopeptides and genetic engineering of Pseudomonas fluorescens.

Email: piotr.biniarz@biotech.uni.wroc.pl