Journal of Vaccines & Vaccination

Construction and immunological study on recombinant multiepitope DNA vaccine of H3/H1subtypes of infl uenza virus

International Conference & Exhibition on Vaccines & Vaccination

22-24 Nov 2011 Philadelphia Airport Marriott, USA

Huijun Lu, Lei Tan, Mingyao Tian and Ningyi Jin

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

Th e eukaryotic expression vector pVAX1 was designed to multi-epitope DNA vaccine mainly on seasonal infl uenza H3 subtype and pandemic infl uenza H1 subtype combined to epitope cassette which have a breakthrough species barrier to infect humans Avian infl uenza H5, H7, H9 subtype HA antigen Th and B cell epitopes, and constructed the single expression of H3HA and H1HA1 the control group. Th e components of antigen transcription and antigenicity were detected by RT-PCR, indirect immunofl uorescence, Western Blot methods. Multi-epitope DNA vaccine was taken immune with mice assisted with electroporation method, and detected the cellular immunity and humoral immunity level of each immunization groups. Humoral immunity results show that with specifi c antigen stimulation, multiple epitopes immunization groups assisted with electroporation got the same level with the single-expression group, and they were signifi cantly higher than the group not using electroporation. Multi-epitope immunization group take an signifi cant advantage on H5/H7/H9 subtypes epitope related specifi c antigen stimulation.IL-2 and IL-4 cytokines detection results, multi-epitope assisted electroporation immunization was signifi cantly higher than that of single expression group and non use electroporation. Use low doses of H1 subtype infl uenza virus on the immune protection of mice to challenge, multi-epitope immunization group assisted electroporation can produce a better immune protection (80%), which is signifi cantly higher than that of single-expression group and non-use of electroporation assisted.

Biography :

Huijun Lu completed his MD. and Ph.D. from AMMS and JLU in China. Dr. Huijun Lu has received many honor and awards. He is a Research Associate at Military Veterinary Institute of Academy of Military Medical Sciences of PLA. He is major in genetic engineering in fl uenza virus vaccine research in China.