Journal of Nanomedicine & Nanotechnology

Concentration dependent internalization and toxicity of iron oxide nanoparticles

4^th International Conference on Nanotek & Expo

December 01-03, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Usha Singh Gaharwar and Paulraj R

Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Ever since the nanotechnology and materials science have emerged, the application of nanomaterials have increased exponentially in many fields including medicine, pharmaceuticals, cell sorting, hyperthermia, cosmetics, or combinations of multiple applications. Iron oxide nanoparticles (IONPs) in particular are increasingly used in medical applications, such as drug delivery, imaging, magneto-fection, tissue repair, cellular therapy and cell labelling. However, toxicity of the IONPs has not been fully elucidated. Hence the present study is aimed to explore the possible interaction of iron oxide nanoparticles and its toxicity on splenic lymphocytes of male Wistar rat. To address the issue we have selected and characterized iron oxide nanoparticles. The average hydrodynamic diameter and shape of nanoparticles were ~35 nm and spherical respectively. Lymphocytes were treated with different concentration of IONPs for different time intervals (24 and 48 h). Exposure of cultured rat?s lymphocytes with IONPs showed a time and concentration dependent uptake of the particles, as demonstrated by transmission electron microscope and elemental mapping (TEM-EM). Higher concentration and longer duration of exposure altered cell viability. TEM images and elemental mapping revealed the adherence of the IONPs to the cell membrane as well as internalization and accumulation in the intracellular vesicles. As the concentration and time increase, densely packed large vesicles were formed in the lymphocytes. In addition, longer period of incubation induced oxidative damage in lipid and DNA. The observation suggests that endocytotic process may involve in uptake which lead to cellular damage.