Journal of Microbial & Biochemical Technology

Comparative study using Maldi Tof MS and automated system in identification of coagulase negative Staphylococci isolated from blood culture

2^nd International Conference on Clinical Microbiology & Microbial Genomics

September 16-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Mustafa Guney

Posters: J Microb Biochem Technol

Abstract:

Accurate identification of coagulase-negative staphylococci (CoNS) is important especially when they are isolated from multiple blood cultures of a patient. Maldi-Tof MS is a new fast and accurate technique which allows examination of protein profiles of bacteria. This study aimed to compare two methods; Maldi-Tof MS (Bruker Daltonics) and BD Phoenix (Becton Dickinson) for identification of CoNS isolates from blood culture. Totally 102 CoNS bloodstream isolates representing 9 species were analyzed in this study. There was 73.5% correlation between two methods. The 10 S.hominis described by Maldi-Tof MS were identified as 3 S.epidermidis , 5 S.saprophyticus , 1 S.capitis , and 1 S.haemolyticus ; 12 S.epidermidis identified as 3 S.saprophyticus , 2 S.haemolyticus 1 S.capitis, 1 S.hominis , 1 S.chromotogenes , 1 S.warneri , 1 S.hyicus , 1 S.aureus , and 1 S.capitis ; 4 S.haemolyticus were identified as 2 S.epidermidis , and 2 S.hominis ; 1 S.warneri was identified as S.capitis by BD Phoenix system. According to the previous studies Maldi-tof MS seemed to be a powerful method in ID of CoNS with up to 99.3% correct results. The next step of this study will be tuf sequencing to find the accuracy rates of these two methods.