Journal of Stem Cell Research & Therapy

Combined efficacy of tamoxifen (TAM) and thymoquinone (TQ) on the lipid peroxidation and the total antioxidant capacity and in DMBA induced mammary carcinoma in female Sprague-Dawleyrats

2^nd International Conference and Exhibition on Cell & Gene Therapy

October 23-25, 2013 Holiday Inn Orlando International Airport, Orlando, FL, USA

Nadiah Alotaibi

Posters: J Stem Cell Res Ther

Abstract:

Reactive oxygen species play a major role in tumor promotion. My aim was to determine the efficacy of combined TAM and TQ on antioxidant status and lipid peroxidation in female rats with DMBA induced mammary tumors. TAM is a drug used to treat women with estrogen sensitive breast cancer, and TQ, from Nigella Sativa oil, is a Middle East natural medicine. My hypothesis was that the combination of TAM and TQ would increase the production of antioxidant enzymesand decreasing the lipid levels more the TAM or TQ alone. Five groups of 10 rats each (control, DMBA, DMBA+TAM, DMBA+TQ, DMBA+TAM & TQ) received DMBA (single dose) followed by daily treatments (TAM, TQ, none), by oral gavage. After 10 wk blood samples were collected by cardiac puncture. The total antioxidant capacity and the lipid peroxidation were measured by OxiSelect assay kits. Data were analyzed by the Kruskal-Wallis test.

Biography :

Nadiah Alotaibi received her Bachelor?s degree in Botany & Microbiology from Dammam University, Saudi Arabia. Now she is a graduate student in the biology department at Eastern Washington University, USA. She is currently studying the combined efficiency of thymouionin andtamoxifen against breast cancer.