Journal of Microbial & Biochemical Technology

Co-immobilization of enzymes: Necessity, problems and some solutions

12^th World Congress on Biotechnology and Microbiology

June 28-29, 2018 | Amsterdam, Netherlands

Roberto Fernandez-Lafuente Sara Peirce , Jose J Virgen-Ortíz, Laura Fernandez-Lopez, Veymar G Tacias-Pascacio and Hadjer Zaak

UAM-CSIC, Spain
CIAD, Mexico

Keynote: J Microb Biochem Technol

Abstract:

Coimmobilization of enzymes is an increasing topic in biocatalysis. It has can save the lag-time that produce the use of enzymes independently immobilized in different particle in casdae reactions. In some cases, is fully compulsory to prevent the destruction of one of the substrates. However, there some problems directly related to the fact of coimmobilizing two enzymes in one particle. First, it is possible that the best immobilization protocol differs from one enzyme to the other, making necessary to reach compromise solutions. This makes that immobilization cannot be fully utilized to get improved preparations of both enzymes, losing positive impacts in the biocatalyst design. Second, after inactivation of the least stable enzyme, both enzymes need to be discarded, even if the other enzyme remains fully active. Here, we presented a solution to these problems, when one enzyme may be stabilized via immobilization, while the other cannot, and the first immobilized/stabilized enzyme is much more stable than the other enzyme. Using as model enzymes a galactosidase and lipases, we will show how we can coat the immobilized/stabilized lipase, then coat the lipase with PEI and finally immobilize the galactosidase. When the galactosidase is inactivated, the lipases remain almost fully active, that way using high buffer concentration the galactosidase may be released. To prevent the PEI release, we have attached it to the enzyme (using glutaraldehyde) and the support (using glyoxyl-octyl). This way, several cycles of lipase reuse after galactosidase inactivation could be performed.

Biography :

Roberto Fernandez-Lafuente has completed his PhD at UAM and Postdoctoral studies from UCL-London. He is leading the “Optimization of biocatalysts and bioprocess” group at ICP-CSIC, a premier Bio-Soft service organization. He has published more than 390 papers in ISI Journal, with an H index of 68 (Scopus) and more than 2200 citations/year, he is co-author of 20 patents and co-supervisor of 18 doctoral thesis. He is an Associated Editor of Process Biochemistry and has been serving as an Editorial Board Member of more than 20 journals (e.g., Enzyme and Microbial Technology, Journal of Biotechnology, Journal of Molecular Catalysis etc.).

E-mail: rfll@icp.csic.esr