Journal of Bacteriology & Parasitology

Classical and Molecular Identification of Lyme Borreliosis agent (Borrelia burgdorferi) in Ixodes ricinus Ticks collected from Northern parts of Iran

2^nd World Congress on Parasitology, Microbiology and Infection Control

November 08-09, 2021 | Webinar

M. Abdigoudarzi

Razi Vaccine and Serum Research Institute, Iran

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Lyme Borreliosis has been directed scientists?? attention around the world during last three decades. It is transmitted by different stages of Ixodes ricinus in Europe and different spp. of Ixodes in North America. As far as Ixodes ricinus is distributed in Northern provinces of Iran which a Mediterranean wet type climate is prevalent, collected ticks from cattle and sheep host of this region were detected by direct examination and molecular methods for Borrelia burgdorferi confirmation. Temporal and spatial identities of Ixodes ricinus were determined. Direct examination on very fresh collected and cold box carried ticks were done using PBS mounted ticks under a dark field microscope or Giemsa stained smears from hemolymph under a compound microscope. A broadly specific primer yielding a 215bp, PCR product was selected. Extracted DNA from individual ticks were subjected to specific PCR and data were collected. Three different confirmed standard DNA of Borrelia and Borrelia burgdorferi have been provided from Japan and Czech, which were used as positive control during PCR trials. Ten groups of collected ticks were under investigation during 2021 and collection of ticks are continued up to March of 2022.

Biography :

M. Abdigoudarzi has completed his BSc from Shiraz University, Master of Science and Public Health from Tehran University of Medical Sciences and PhD from Tarbiat Modares University, Finalized a Thesis on Hyalomma molecular Taxonomy at Pasteur Ins. Of Tehran. He was a lecturer on ticks and tick borne diseases in Tunisia, Swiss, Turkey, Romania and granted a joint project study at Germany (Borstel Research Inst.). He published many sequences of specific DNA for Ticks in Pubmed. He has published more than 50 papers in reputed journals.