Journal of Vaccines & Vaccination

Chicken bone marrow-derived dendritic cells induced by a Salmonella vaccine candidate

9^th Global Summit and Expo on Vaccines & Vaccination

November 30-December 02, 2015 San Francisco, USA

John Hwa Lee

Chonbuk National University, Republic of Korea

Posters-Accepted Abstracts: J Vaccines & Vaccin

Abstract:

We aimed to evaluate immune-stimulatory effect of Salmonella enteritidis ghost vaccine on maturation of chicken bone marrow-derived dendritic cells (BM-DCs) in vitro. The immature BM-DCs were stimulated with vaccine. The vaccine efficiently stimulated maturation events in BM-DCs, indicated by up-regulated expression of CD40, CD80 and MHC-II molecules. Immature BM-DCs responded to stimulation with vaccine by expression of TNF-α, IL-6 and IL-12 cytokines. In addition, vaccine stimulated BM-DCs could induce significant expression of IFN-γ and IL-2 in co-culture with autologous lymphocytes. In conclusion, our data suggest that vaccine candidate is capable of activating DCs for modulation of adaptive immune responses.

Biography :

John Hwa Lee is a professor and Dean at the Chonbuk National University, College of Veterinary Medicine, South Korea. He has a Ph.D. in Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, USA. He is an Editorial Board for Food borne Pathogens and Disease and Associate Editor for Journal of Veterinary Science.

Email: johnhlee@jbnu.ac.kr