Journal of Nanomedicine & Nanotechnology

Cellulose derivative-based polymeric nanoparticles for Garciniamangostana extract delivery and cancer therapy

2^nd International Conference on Nanotek and Expo

December 3-5, 2012 DoubleTree by Hilton Philadelphia Center City, USA

Porntip Pan-in, Anthony J. Kim, Justin Hanes and SupasonWanichwecharungruang

Posters: J Nanomed Nanotechol

Abstract:

Cervical cancer is an important health problem for women worldwide. Although chemotherapeutic agents are a widely used in cancer treatments, they can leadto high toxicity and harmful side effects. To avoid side effects of chemotherapeutics, the use of natural phytochemicals such as Xanthone from Garciniamangostana extract (GME) has received considerable attention in cancer prevention and cancer therapeutics. However, GME?spoor aqueous solubility and low bioavailability are the major obstacles toward its development. Encapsulation using polymeric nano carriers is one of the ways to overcome the problem of GME. Among the polymers suitable for encapsulation, cellulose derivative, ethyl cellulose (EC) and methylcellulose (MC), are non-toxic, stable, and have been widely used in pharmaceutics. We prepared GME-encapsulated polymer-blend of EC and MC nanoparticles (nanoGME) using a solvent displacement method with high encapsulation efficiency and size around 250 nm. In vitro cytotoxicity assay showed effective anticancer activity of nanoGME in HeLa cells. Moreover, uptake of nanoGME by HeLa cells was higher than unencapsulated GME and nanoGME, which entered cells via clathrin-mediated endocytosis. As far as we know, this is the first report on the intracellular pathway of cellulose derivative nanoparticles. In summary, NanoGME successfully improved the bioavailability of GME in aqueous solution and enhanced cellular uptake

Biography :

Porntip Pan-inis a Ph.D. student in Biotechnology program at the Faculty of Science, Chulalongkorn University. Pan-in?s current Ph.D. thesis is in drug and natural product delivery for bacterial infection and cancer therapy under the supervision of Dr. Supason Wanichwecharungruang, a professor at Chulalongkorn University.She is currently a trainee at Johns Hopkins University investigating intracellular delivery of nanoparticles and gene therapy under Dr. Justin Hanes, a professor at Johns Hopkins University. She is also interested in the commercial application of her natural product delivery using nanopartcles research