Pharmaceutica Analytica Acta

Cell incorporation studies with ^99mTc-Methotrexate loaded chitosan nanoparticles for breast cancer diagnosis

8^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 07-09, 2016 Madrid, Spain

Meliha Ekinci, Derya Ilem-Ozdemir, Evren Gundogdu and Makbule Asikoglu

Ege University, Turkey

Scientific Tracks Abstracts: Pharm Anal Acta

Abstract:

The aim of this study is to evaluate newly developed radiopharmaceuticalâ??s (99mTc-MTX-CSNPs) incorporation to human breast cancer (MCF-7) and human keratinocyte (HaCaT) cell lines for breast cancer diagnosis. For this purpose, methotrexate (MTX) loaded chitosan nanoparticles (CSNPs) were prepared by ionic gelation process, stored for 6 months and evaluated in terms of particle size, PdI value and zeta potential. Produced MTX-CSNPs were radiolabeled by 99mTc with high labeling efficiency (>90%). Then, newly developed radiopharmaceuticalâ??s target/non-target ratio was investigated with cell culture studies by using MCF-7 and HaCaT cell lines. Results demonstrated that the incorporation of 99mTc-MTX-CSNPs in breast cancer cells was found about 2-times higher than normal cells. So, 99mTc-MTX-CSNPs might be used for human breast cancer diagnosis in nuclear medicine patients.

Biography :

Meliha Ekinci received her Master’s degree in Radiopharmacy from Ege University (Turkey) in 2015. She is currently a Research Assistant at Radiopharmacy Department and also a Doctoral student at Biopharmaceutics and Pharmacokinetics Department, Ege University. She has already published 4 papers in reputed journals. Her research interest is in radiolabeled and evaluated newly developed radiopharmaceuticals for diagnosis of different cancer types.

Email: meliha.ekinci@ege.edu.tr