Journal of Stem Cell Research & Therapy

CD₂₂₆ and CD₄₀ gene polymorphism in susceptibility to juvenile rheumatoid arthritis in Egyptian patients

5^th International Conference and Exhibition on Cell and Gene Therapy

May 19-21, 2016 San Antonio, USA

Mosaad H (Heba Mosaad)

Mansoura University School of Medicine, Egypt

Scientific Tracks Abstracts: J Stem Cell Res Ther

Abstract:

Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease of the childhood with a high risk of disability in Egyptian children. JIA has many genetic factors affecting its pathogenesis including CD226 and CD40 genes. These genetic factors vary in different races proved by previous studies on European and Chinese populations. The association between JIA and CD226 and CD40 is yet to be evaluated in non-European populations including Egypt. So we studied the association of CD226 rs1883832 (-1C>T) and CD40 rs1883832 (-1C>T) gene polymorphism and disease susceptibility and severity of in an Egyptian cohort. Subject & Methods: In this case control study we recruited 79 Egyptian children with JIA and 93 healthy controls. We studied CD226 rs763361 (C>T) using the tetra amplification refractory mutation system - polymerase chain reaction assay (ARMS-PCR) for detection of polymorphism while for CD40 rs1883832 (-1C>T) we used restriction fragment length polymorphism (RFLP). Results: The statistical results showed that the rs763361 (C>T) SNP in the CD226 gene is significantly associated with JIA group as regard to TT genotypes (p= 0.0001). The frequency of the T allele was significantly higher in JIA patients in comparison with the control group (p= 0.0001). Also this allele was significantly higher in patients with moderate and sever JIA when compared to controls (p=0.003). This allele correlated to the disease severity (OR=2.4). Study of CD40 rs1883832 (-1C>T) showed that the distribution of the C allele was significantly higher in JIA patients (p=0.003). Also it was significantly higher in patients with moderate and sever JIA when compared to controls (p=0.01). Conclusion: These results demonstrate a genetic association between the CD226 and CD40 gene polymorphism and JIA with an impact on disease severity in an Egyptian cohort.

Biography :

Email: mosaad_heba@yahoo.com