Journal of Bioequivalence & Bioavailability

Bioanalytical method development and validation for bioflavonoid quercetin in nano formulation by RP-UFLC in rabbit plasma

4^th World Congress on Bioavailability and Bioequivalence: Pharmaceutical R&D Summit

May 20-22, 2013 DoubleTree by Hilton, Beijing, China

R Shanmugam

Posters: J Bioequiv Availab

Abstract:

A sensitive reverse phase high pressure liquid chromatography (RP-HPLC) has been developed and validated for the quantification of quercetin in rabbit plasma using protein precipitation method (PPT). Best chromatographic resolution was achieved on a reverse phase Hibar C 18 (250 x 4.6 mm i.d., 5μ) column with the mobile phase consisted of acetonitrile: ammonium acetate buffer (pH 3.5) in the ratio of 75:25 (v/v) with a flow rate of 0.8ml/min. The retention time for the drugs was found to be 5.6 minute and 10 minute for internal standard (fluticasone propionate). The analyte was detected by using a PDA detector with LC solution software. Linearity was obtained in the concentration range from (20 to 400.0 ng/ml) (r = 0.989). Lower limit of quantification (LLOQ) was found to be 10 ng/ml. Middle limit of quantification (MQC) was found to be 200 ng/ml and higher limit of quantification (HQC) was found to be 400 ng/ml. The average recovery of the analyte was found to ranging from 95.82 to 98.8%. The present method was successfully applied in the newly developed nano-formulation for estimation of various pharmacokinetic parameters such as peak plasma concentration (C max ), time to peak concentration (t max ), area under the plasma concentration-time curve (AUC 0-t & AUC 0-∞ ), elimination rate constant (k el ) and elimination half-life (t 1/2 ) for quercetin in rabbit plasma