Brain Disorders & Therapy

Astrocytic gap junctions contribute to potassium redistribution over the neocortex

6^th International Conference on Brain Disorders and Therapeutics

September 13-15, 2018 | Copenhagen, Denmark

Azin Ebrahim Amini

University of Toronto, Canada

Posters & Accepted Abstracts: Brain Disord Ther

Abstract:

Extracellular potassium ion concentration ([K]e) is tightly regulated throughout the brain because it has a major impact on brain functionality. Potassium concentration is disrupted in many brain diseases such as stroke and epilepsy. This project is designed based on a well-developed experimental platform to investigate the effects of extracellular potassium redistribution in physiological states. All experiments were conducted in vivo in mouse neocortex. Two double-barreled K-sensitive electrodes coupled with local field potential (LFP) electrodes were placed 4 mm apart. 50 mM KCl solution was injected focally closer to one of the K-LFP electrodes. [K]e levels and LFP were measured in two different scenarios: pharmacological intervention (gap junction blockage) and optical intervention (optogenetics). Focally increased [K]e was associated with a transient depolarization which in turn spreads into neighboring tissues so called spreading depolarization. Gap junctional blockade in the peri-injection site simultaneously increased the amplitude and duration of the local [K]e response, and the local field response was greatly prolonged. While in the remote injection site, [K]e response was decreased after gap junctional blockage application. Optical stimulation decreased the [K]e both in the peri-injection and remote site. Our preliminary results are evidence of slow K redistribution (take for minutes) throughout the astrocytic syncytium which is partly mediated via astrocytic gap junctions. Potassium redistribution across a large area of the cortex is not a well-studied area because most studies have limited their focus on focal potassium dynamics. In this project we are addressing this gap using novel tools to elucidate potassium redistribution dynamics.

Biography :

E-mail: azin.amini@mail.utoronto.ca