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Association between single nucleotide polymorphism +874 A/T and its susceptibility to pediatric tuberculosis in Indonesia
International Conference on Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
Rini Savitri Daulay, Ridwan Muchtar Daulay, Ratna Akbari Ganie, Gino Tann and Bambang Supriyatno
University of Sumatera Utara, Indonesia
University of Indonesia, Indonesia
Posters & Accepted Abstracts: Biochem Anal Biochem
Abstract:
Tuberculosis (TB) is one of the leading causes of morbidity and mortality worldwide especially, in developing countries. TB is a complex multifactorial disease with genetic as one of the substantial factors for TB development. Our hypothesis is that single nucleotide polymorphism (SNP) +874 A/T affects low production of IFN-γ level that increased susceptibility of pediatric TB. The aim of this study was to investigate association between SNP +874 A/T and its susceptibility to pediatric TB in Indonesia. DNA samples were obtained from 50 patients with pulmonary TB, 1 patient with extra pulmonary TB and 51 healthy controls. SNP +874 A/T was identified using the amplification refractory mutational system polymerase chain reaction (ARMS-PCR) method. The result of this study showed the presence of AA, AT and TT genotype in TB patients were 31 (60.8%), 20 (39.2%) and 0 (0%); respectively (p=0.023). Significant decreased in production of IFN-γ level (p=0.042) was found in TB patients (10.49±6.26 pg/ml) which contrast to healthy controls (10.80±14.48 pg/ml). Low production of IFN-γ level was identified among AA genotype patients (10.44±8.24 pg/ ml) compared to AT genotype patients (11.17±13.71 pg/ml), but not significantly proven. An allele was found to be a risk factor for development of TB disease (OR, 1.51; 95% CI=1.04-2.21, p=0.018). In conclusion, this study has provided evidence of the association between SNP +874 A/T and its susceptibility to pediatric TB. AA genotype and an allele were found significant among pediatric TB patients in Indonesia.
Biography :
Email: rini.daulay@gmail.com