Oral Health and Dental Management

Antioxidant and cytotoxicity activity of Areca nut (Areca catechu L.) extract as natural anticancer agent for oral squamous cell carcinoma

28^th Asia Pacific Congress on Dental and Oral Health

July 10-12, 2017 Kuala Lumpur, Malaysia

Liza Meutia Sari, Gus Permana Subita and Elza Ibrahim Auerkari

Syiah Kuala University, Indonesia

Posters & Accepted Abstracts: Oral Health Dent Manag

Abstract:

Many herbs have been discovered to be potential sources of anticancer drugs. Biji Pinang or Areca nut has a high content of phenolics and flavonoids and which is related to antioxidant activity. However, data on its effects on oral squamous cell carcinoma, human keratinocyte cells and the safety of its use is not available. The objective of this study is to identify the phytochemical composition of Areca nut, antioxidant activity and cytotoxicity activity in HSC-2, HSC-3 and HaCat. The Areca nut was extracted by ethanol 96%, phytochemical screening, LC-MS, DPPH assay and MTS assay. The presence of alkaloid, tannin, flavonoid, catechin and quercetin compounds in Areca nut extract. Total phenolic content was 80.3 mg TAE/g and total flavonoid content was 238.5 mg CE/g. The EC50 was 15.95±0.84 μg/ml. The extract of Areca nut showed higher toxicity on HSC-3 cell compared to HSC-3 IC50 which was 164.06 μg/ml vs. 629.50 μg/ml in HSC-2.The proliferation effect was found in HaCat cells. The antioxidant activity of the extract of Areca nut might be associated with the presence of catechin, selective cytotoxicity on HSC-2, HSC-3 and HaCat cells.

Biography :

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