Pharmaceutica Analytica Acta

Anti-MRSA activity of cationic lipid carriers in combination with oxacillin for eradicating skin infection

International Conference on Bio-Pharmaceuticals, Biosimilars & Pharma Industry

February 28 - March 01, 2019 Osaka, Japan

Jia-You Fang, Ya-Ping Chen and Shih-Chun Yang

Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan Department of Cosmetic Science, Providence University, Taichung, Taiwan

Scientific Tracks Abstracts: Pharm Anal Acta

Abstract:

Nanoparticles have been the focus of being the antibacterial agents. The aim of this study was to evaluate the anti-methicillinresistant Staphylococcus aureus (MRSA) activity of cationic nanostructured lipid carriers (NLC) combined with oxacillin against ATCC 33591 and clinical isolate. The cationic resource on NLC surface was the decoration of soyaethyl morpholinium ethosulfate (SME). NLC loaded with oxacillin was produced to assess antibacterial activity and the topical application for treating cutaneous infection. The hydrodynamic diameter and zeta potential of oxacillin-loaded NLC were 177 nm and 19 mV, respectively. Oxacillin exhibited synergistic MRSA eradication when combined with NLC. The minimum bactericidal concentration (MBC) of oxacillin was decreased from 250 to 62.5 μg/ml after NLC encapsulation. The combined NLC and oxacillin reduced MRSA biofilm thickness from 31.2 to 13.0 μm, which was less than NLC (18.2 μm) and antibiotic (25.2 μm) alone. The oxacillin-loaded NLC showed a significant reduction in the burden of intracellular MRSA in differentiated THP-1 cells. This reduction was greater than that of individual treatment. The mechanistic study demonstrated the ability of cationic NLC to disrupt bacterial membrane, leading to the protein leakage. The cell surface disintegration also increased oxacillin delivery into the cytoplasm for activating the bactericidal process. Topical NLC treatment of MRSA abscess in skin decreased bacterial load by 4 logarithm and improved skin architecture and barrier function. Our results demonstrated that a combination of nanocarriers and an antibiotic could synergistically inhibit MRSA growth. It can be a potential approach to resolve the risk of drug resistance.

Biography :

Dr. Jia-You Fang is a Professor in the Graduate Institute of Natural Products at Chang Gung University in Taiwan. In addition, he is an Adjunct Professor in the Graduate Institute of Health Industrial Technology, Chang Gung University of Science and Technology and Department of Anesthesiology, Chang Gung Memorial Hospital. Over the past 25 years, he and his team have made outstanding contributions to the understanding of drug delivery, pharmaceutics, pharmacokinetics, nanomedicine, and cosmetology. His contributions add up to 271 peer-review articles, more than 6000 citations and H index of 45.

E-mail: fajy@mail.cgu.edu.tw