Journal of Microbial & Biochemical Technology

Antimicrobial, anticoagulation, fibrinolytic and prebiotic activities of exopolysaccharide produced by marine Klebsiella spp.

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Ayman Kamal El Essawy

Ain Shams University, Egypt

Scientific Tracks Abstracts: J Microb Biochem Technol

Abstract:

The authors previously, achieved a high yield of exopolysaccharide production from a marine Klebsiella spp. The aim of the present study is determination of this exopolysaccharide composition and evaluation of its biological activities. The monosaccharide moieties of this exopolysaccharide were determined by complete hydrolysis, qualitative and quantitative paper chromatography of the hydrolysis product. The monosaccharide composition of this exopolysaccharide was galactose 16%, fructose 20%, glucose 32%, fucose 22%, and uronic acid 10%. The biological activities of the native and modified sulfated bacterial exopolysaccharide were investigated. No anticoagulation or fibrinolytic activities were recorded for the sulfated exopolysaccharide, but the native form of exopolysaccharide showed both activities. The native exopolysaccharide also showed higher antibacterial activity against S. aureus and E. coli but both the native and sulfated form had no antifungal activity against Candida albicans. MIC of the native and sulfated exopolysaccharide that sustain the highest bacterial inhibition zone was 15 mg/dl. No prebiotic activities were recorded for both. In conclusion, this biopolymer has significant biological activities and it has two rare monosaccharide moieties with very significant value namely fucose and uronic acid that require further adaptation for practical and industrial application.

Biography :

Ayman Kamal El Essawy has completed his PhD in Microbiology from Ain Shams University, Egypt, Diploma in Hospital Infection Control at Claude Bernard-Lyone 1 University, France and a Diploma in Biostatistics from Ain Shams University, Egypt. He is fellow of Microbiology at Ain Shams University. He worked at Al Azhar University, American Naval Medical Research Unit No.3 (NAMRU-3) and Ain Shams University Genetic Engineering/Biotechnology center. He is publishing mainly in the field of Microbiology and Molecular Microbiology. He is particularly interested in the study of bacterial resistance to antibiotics.