Journal of Microbial & Biochemical Technology

Antimicrobial and antioxidant activity of L-asparaginase producing marine Streptomyces mutabilis

International Conference and Summit on Industrial & Pharmaceutical Microbiology

October 17-18, 2016 Kuala Lumpur, Malaysia

P Ayyanar

Bharathidasan University, India

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Marine ecosystem consisting vast number of biodiversity and among thus actinomycetes is playing an important role for drug discovery. Many existing drugs are derived from marine actinomycetes. Well known actinomycetes like Streptomyces, Actinomyces, Arthrobacter, Corynebacterium, Frankia, Micrococcus, Micromonospora are representative genus and several others. Metabolites of actinomycetes possess a wide range of biological activities like antimicrobial, antitumor, immunosuppressive, antileukemic, anti-VRE, telomerase inhibitor, Antihelminthic, cell growth inhibitor, herbicidal, apoptosis inducer, neuritogenic activity, antimalarial, antitrypanosomal and antileshmanial. L-asparaginase (EC 3.5.1.1) is a naturally occurring enzyme that catalyzes the hydrolysis of asparagine to aspartic acid. The FDA has approved L-asparaginase for the effective treatment of acute lymphoblastic leukemia and lymphosarcoma. In this present investigation, the marine sediment sample was collected from Cochin, India. The actinomycetes strains were isolate and screened for L-asparaginase and better strains was taken for further identification at molecular level using 16s rDNA technique and it was identified as Streptomyces mutabilis and was named as strain INR 1 (KT887962). Antibacterial activity was proved using primary and secondary method against B. megaterium, S. epidermitis, E. coli, S. flexneri, S. Typhi, V. cholerae, K. pneumonae and P. aeroginosa and as well as FTIR characterization of crude protein showed the presence of functional groups of protein like NH, COOH, -CO-, CH, SH, C=O, C=C and other R groups at appropriate wave length and stretching. Also the free radical scavenging activities were accessed by Hydroxyl, DPPH, ABTS, RP, NO and SOD radicals. This study concludes the strain Streptomyces mutabilis shows good L-asparaginase production, antibacterial activity against human bacterial pathogens and free radical scavenging activity. This strain might control the cancer cells proliferation as well as microbes.

Biography :

Email: ayyanarpandurangan@gmail.com