Journal of Food Processing & Technology

Anti-adipogenic activity of Australian canola meal in murine mesenchymal stem cells

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Saira Hussain, Padraig Strappe, Ata-ur-Rehman, David Luckett and Christopher L Blanchard

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Obesity is associated with many diseases including cardiovascular diseases, diabetes, atherosclerosis, hypertension and cancer.
Potential bioactive compounds from plant extracts may inhibit fat cell (adipocyte) differentiation. Screening of such extracts
may contribute to future novel functional food strategies reduce obesity. Canola meal is a waste product left after extraction of the oil
and may be a source of potential bioactive compounds which may inhibit fat cell development. In this study, canola meal extracted
using a variety of solvents was examined for its ability to suppress adipocyte differentiation in C3H10T1/2 murine mesenchymal stem
cells. Accumulation of fat within the C3H10T1/2 cells was measured through Oil Red O staining while levels of the major adipocyte
differentiation factor PPARγ, were analysed by immunofluorescence and quantative polymerase chain reaction (qPCR). Overall
extracts of canola meal demonstrated significant inhibition of adipocyte differentiation without cell toxicity with acetone and hexane
extracts being the most effective.