Journal of Stem Cell Research & Therapy

Angiogenic potential of decellularized rat esophageal scaffolds in vivo

11^th Annual Conference on Stem Cell and Regenerative Medicine

October 15-16, 2018 Helsinki, Finland

Elena Gubareva, Elena V Kuevda, Ramazan Z Nakokhov, Dmitry P Puzanov, Timofei E Grigoriev, Ilya M Bykov, Peter G Storozhuk, Alexander L Vasiliev and Sergei N Chvalun

Kuban State Medical University, Russia
Kurchatov Institute, Russia

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

Currently, the only existing method of treatment for esophageal medical conditions such as tumors, trauma or congenital abnormalities is surgical resection and reconstruction, usually using replacement tissue taken from the patients??? stomach, colon or jejunum and associated with considerable morbidity and mortality. Tissue engineering represents a promising alternative, where 3-dimensional biological acellular scaffolds provide the ability to support cell viability, proliferation, angiogenesis and cues to allow tissue regeneration. In our research we describe esophageal decellularized matrices to promote vascularization in vivo. This study was performed on 30 male Wistar rats weighting 220±50 g at the Laboratory of Basic Research in the field of regenerative medicine, Kuban State Medical University (Krasnodar). Acellular rat esophagus matrices were obtained after detergent-enzymatic decellularization with 4% sodium deoxycholate, purified deionized water, porcine pancreatic DNAse I and phosphate buffer saline during 21 hours at the perfusion rate of 6 mL/min. The scaffolds quality was assessed according to the grade intensity of structural changes in the matrices after routine histological staining procedures and immunohistochemistry, which were also used to confirm the presence of cells on the top and inside the scaffold and revascularization after explantation. The acellular esophagi were implanted both subcutaneous and orthotopic in rats. Orthotopic transplantation was performed as a patch graft technique the native esophagus was dissected one-third and a portion of the host esophagus was replaced with decellularized graft. Heterotopic transplanted scaffolds were vascularized in 7 days after implantation. Orthotopic implantation of decellularized esophageal matrices promoted newly formed blood vessels with cells visible inside scaffolds and positive to CD31, VEGF, vWF. In conclusion, our study suggested that acellular esophageal scaffold has angiogenic potential and pro-regenerative environment activated in vivo and appears to be a promising challenge for esophagus engineering and reconstruction.

Biography :

Elena Gubareva has her expertise in Regenerative Medicine and Tissue Engineering. She has completed her MD and the PhD from Kuban State Medical University. She works as Laboratory Head in the Laboratory of Fundamental Research in the field of regenerative medicine, Kuban State Medical University, Russia. She has published more than 100 papers in reputed journals.

E-mail: g_lena82@list.ru