Journal of Pharmacogenomics & Pharmacoproteomics

An implantable lactate biosensor based on the co-electrodeposition of lactate oxidase and a low-potential redox polymer

5^th International Conference on Predictive, Preventive and Personalized Medicine & Molecular Diagnostics

December 01-02, 2016 Valencia, Spain

Pin Li, Yan Liu and Zhiqiang Gao

Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
Department of Chemistry, National University of Singapore, Singapore

Posters & Accepted Abstracts: J Pharmacogenomics Pharmacoproteomics

Abstract:

Detection of lactate levels plays a significant role in clinical analysis, sport medicine and food industry. In this work, an implantable lactate biosensor for continuously monitoring lactate levels in vivo was fabricated based on the co-electrodeposition of lactate oxidase (LOX) and an osmium-containing low-potential redox polymer (PVI-PAA-Os) on a carbon electrode. The PVI-PAA-Os redox polymer with a low reduction potential and LOX were able to be irreversibly deposited on the surface of the carbon electrode via simple electrochemical techniques such as potential cycling and controlled potential electrolysis. The co-electrodeposition conditions were investigated in terms of pH, PVI-PAA-Os concentration, LOX concentration and the number of deposition cycles. The characteristics of the biosensor for the detection of lactate were thoroughly investigated. It was observed that the co-deposited LOX retains its enzymatic activity and the redox polymer is able to electrochemically mediate electron-transfer between the carbon electrode and the co-deposited LOX. It was also observed that the optimized lactate biosensor exhibits a lower operating potential of +0.15 V (vs. Ag/AgCl) with a detection limit of 10 μM and a linear dynamic range up to 20 mM. In addition, stable responses were obtained over an extended period of seven days of in vivo testing.

Biography :

Email: chmgaoz@nus.edu.sg