Journal of Microbial & Biochemical Technology

Age-related mitochondrial dysfunction influences the mouse intestinal microbiome composition

International Conference and Summit on Industrial & Pharmaceutical Microbiology

October 17-18, 2016 Kuala Lumpur, Malaysia

David Houghton, Christopher J Stewart, Craig Stamp, Andrew Nelson, Nadim J Ajami, Joseph F Petrosino, Anil Wipat, Michael I Trenell, Douglass M Turnbull and Laura C Greaves

Newcastle University, UK
Baylor College of Medicine, USA
Northumbria University, UK

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Statement of the Problem: Mitochondrial dysfunction occurs as the gastrointestinal tract ages. We investigated whether changes in mitochondrial function in ageing colonic crypts in mice with or without mitochondrial defects influence the microbial gut communities and whether exercise modulates any such changes. Methodology: 12 PolgAmut/mut mice and 7 age matched wild type PolgA+/+ mice were used in the current study. The 12 PolgAmut/ mut mice were randomly divided into a sedentary and exercise group at 4 months and PolgA+/+ remained sedentary throughout. Stool samples were collected at 4, 7 and 11 months and bacterial profiling was achieved through 16S rDNA sequencing profiling. Mitochondrial enzyme activity was assessed in colonic epithelial crypts at 11 months for PolgAmut/mut and PolgA+/+ mice. Results: Sedentary and exercise PolgAmut/mut mice had significantly greater mitochondrial dysfunction than PolgA+/+ mice (78%, 77% and 1%, respectively). Bacterial profiles of sedentary PolgAmut/mut mice were significantly different from the sedentary PolgA+/+ mice with increases in Lactobacillus and Mycoplasma and decreases in Alistipes, Odoribacter, Anaeroplasma, Rikenella, Parabacteroides, Allobaculum in the PolgAmut/mut mice. Exercise did not have any impact upon gut mitochondrial dysfunction, however, exercise did increase gut microbiota diversity and significantly increasing bacterial genera Mucispirillum and Desulfovibrio. Conclusion: Mitochondrial dysfunction is associated with changes in the gut microbiota. Endurance exercise moderated some of these changes, establishing that environmental factors can influence gut microbiota despite mitochondrial dysfunction.

Biography :

Email: david.houghton@ncl.ac.uk