Journal of Stem Cell Research & Therapy

A single use platform for expansion of cellular therapeutics

5^th International Conference and Exhibition on Cell and Gene Therapy

May 19-21, 2016 San Antonio, USA

Julie Murrell, Aletta Schnitzler, Susan Rigby, Daniel Kehoe, Manjula Aysola, Sandhya Punreddy, Anjali Verma, Tristan Lawson, Allen Feng and Martha Rook

EMD Millipore Corporation, USA

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

Human cellular therapies are used extensively in clinical studies and as drug discovery tools. Current culture methods involve cumbersome multilayer flatbed culture and roller bottle systems. Several groups previously demonstrated an expansion paradigm that uses a scalable, single use, stirred tank bioreactor for cell expansion. A bioreactor system enables direct monitoring for the specific cell characteristics at any point during the expansion, thus assuring product quality and consistency. In addition, a bioreactor provides ease of use in handling and lower medium volume requirements. Cells cultured in bioreactors were evaluated to assess a variety of markers that confirm identity and purity. No differences were observed in protein markers. Finally, we evaluated concentration technologies and found that good recovery of viable cells is achievable. In this work, we verified that the cells expanded in the single use stirred tank bioreactor and subsequently harvested were identical to those grown in traditional systems.

Biography :

Email: julie.murrell@emdmillipore.com