Journal of Nanomedicine & Nanotechnology

A facile biosensor system for separation and detection of Norovirus using specific binding affinity probes

24^th World Congress on Nanomaterials and Nanotechnology

July 12-13, 2018 Bangkok, Thailand

Tae Jung Park

Chung-Ang University, South Korea

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Norovirus is one of the worldwide infectious diseases caused by eating raw foods which were infected. It is a major cause of foodborne and nosocomial outbreaks. In this study, thorough and highly sensitive biosensors for detecting Norovirus by applying a recognized affinity peptide as a platform were described. These electrochemical and optical methods are costeffective, fast responsive and easy to integrate information into miniaturized micro-devices like a portable biosensor device. The performance of the peptides has been studied with fluorescent optical assay and gold-immobilized synthetic peptides have been studying cyclic voltammetry, impedance spectroscopy and colorimetry analysis. We found that several kinds of peptides (Noro-BP, nonFoul, (FelxL)2, nonFoul(FlexL)2) are the efficient recognizers for Norovirus screened by using the M13 phage display method. These peptides were effectively applied to the analytical methods to detect the real Norovirus sample with Rotavirus as a negative control. Among them, nonFoul (FlexL)2 shows the best performance as the lowest detection limit value of 1.7 copies/mL. In addition, the result could be expected to be useful to peptide-based detection sensor for the Norovirus by using nano-flowers with large surface area. These results suggest that the biosensor consists of specific binding peptide, has affinity to Norovirus as a molecular binder and will be used as a diagnostic tool. Moreover, the biosensor could help as a new bio-sensing platform for point-of-care testing by applying much more fields.