Journal of Bacteriology & Parasitology

A Comparative Study of Gene Expression Among the Different Developmental Stages of Schistosoma mansoni

2^nd World Congress on Parasitology, Microbiology and Infection Control

November 08-09, 2021 | Webinar

Noura Mostafa Kamel Abdelhamid Shebl

Alexandria University, Egypt

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Being a worldwide neglected tropical disease, schistosomiasis mansoni has been in the focus of molecular research. Emergence of drug resistance to praziquantel and its ineffectiveness against immature stages led to the quest of finding novel targets for therapy and vaccinations. The aim of the current study was to compare the expression of three transcripts (lactate dehydrogenase (LDH), aspartic protease (AP), tegumental phosphodiesterase SmNPP-5 (TP)) among different developmental stages in S. mansoni (cercaria, schistosomula, adult male and female worms at different durations of oviposition). The current molecular study was carried out by using SYBR green RT-qPCR. Cercaria were obtained by light shedding technique, 7-days old schistosomulae were obtained from lungs of infected mice, 28-days old mixed adult worm, 38-days and 48-days old adult male and female worms were obtained from infected mice by aseptic portal perfusion. 18S ribosomal RNA was used as the housekeeping gene to calculate relative fold differences by the Rotor Gene software. The results showed that there was an overall fold reduction in the expression of LDH (0.64 ± 0.08) and AP (0.27 ± 0.03) as the parasite develops in comparison to cercariae. TP was absent in the cercarial stage and markedly expressed in schistosomulae. However, in 28-days adult worms TP was highly expressed compared to schistosomulae (1.16-fold) followed by reduction in gene expression pattern (0.9-fold ± 0.04) in the following stages. Statistical analysis of all the collected data revealed that AP was the most abundant transcript throughout the various stages and should be regarded as the most probable target for future schistosomiasis treatment and control.

Biography :

Noura Mostafa Kamel Abdelhamid Shebl has graduated from IGCSE in 2009. She received her Bachelor degree in Medicine and Surgery from Faculty of Medicine, Alexandria University in 2016. Three years ago, she was hired as a teaching assistant in the department of Medical Parasitology, Faculty of Medicine, Alexandria University. Currently she is preparing her master thesis in the field of molecular parasitology.