Oral Health and Dental Management

3D printed polycaprolactone scaffold mixed with tricalcium phosphate as a bone regenerative material in rabbit calvarial defects

26^th Euro Congress and Expo on Dental & Oral Health

December 10-11, 2018 | Rome, Italy

Jeong-Won Paik, Hyung-Chul Pae and Seong- Ho Choi

Yonsei University, South Korea

Posters & Accepted Abstracts: Oral Health Dent Manag

Abstract:

Defect-specific bone regeneration using 3-dimensional (3D) printing of block bone has been developed. Polycaprolactone (PCL) is biocompatible polymer that can be used as 3D scaffold. The aim of this study is to assess biocompatibility and osteogenic efficacy of 3D printed PCL scaffold and to evaluate effectiveness of ?-tricalcium phosphate (?-TCP) addition in PCL scaffold. In this work, four circular defects (diameter: 8mm) in rabbit calvarium were randomly assigned to negative control (control); PCL block (PCL); PCL mixed with 10 wt% ?-TCP (PCL/?-TCP), and; PCL/?-TCP plus collagen membrane (PCL/?-TCP+M). Animals were euthanized at 2 (n=5) and 8 weeks (n=5). Results indicated that in micro-CT, PCL/?-TCP+M showed the highest total augmented volume and new bone volume at 8 weeks, but there was no significant difference among four groups. Histomorphometrically, PCL, PCL/?-TCP, PCL/?-TCP+M showed the significantly higher total augmented area compared to the control. PCL/?-TCP+M showed the highest new bone area, but not statistically higher than the control. New bone formation deep inside the scaffold was observed only in ?-TCP added scaffold. PCL showed high biocompatibility with great volume maintenance. Addition of ?-TCP to PCL seemed to increase hydrophilicity and osteoconductivity. Developments in 3D printed PCL material are expected. Recent Publications 1. Kim J W, Choi K H, Yun J H, Jung U W, Kim C S, Choi S H and Cho K S (2011) Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 in rat calvarial defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 112(3):298-306. 2. Hwang J W, Park J S, Lee J S, Jung U W, Kim C S, Cho K S, Lee Y K and Choi S H (2012) Comparative evaluation of three calcium phosphate synthetic block bone graft materials for bone regeneration in rabbit calvaria. J Biomed Mater Res B Appl Biomater. 100(8):2044-2052. 3. Lee J S, Lee J S, Kang M H, Jung U W, Choi S H and Cho K S (2018) Proof-of-concept study of vertical augmentation using block-type allogenic bone grafts: a preclinical experimental study on rabbit calvaria. J Biomed Mater Res B Appl Biomater 106(7):2700-2707. 4. Rasperini G, Pilipchuk S P, Flanagan C L, Park C H, Pagni G, Hollister S J and Giannobile W V (2015) 3D-printed bioresorbable scaffold for periodontal repair. J Dent Res 94(9 Suppl):153S-157S. 5. Yao Q, Wei B, Guo Y, Jin C, Du X, Yan C, Yan J, Hu W, Xu Y, Zhou Z, Wang Y and Wang L (2015) Design, construction and mechanical testing of digital 3D anatomical data-based PCL-HA bone tissue engineering scaffold. J Mater Sci Mater Med 26(1):5360.

Biography :

Jeong-Won Paik is a Research Professor at Department of Periodontology, College of Dentistry in Yonsei University.

E-mail: jpaik@yuhs.ac