Pharmaceutica Analytica Acta

The hyphenated technique is developed from the coupling of a separation technique an on-line spectroscopic detection technology. Mainly chromatographic techniques are combined with spectroscopic techniques. Then the separated components of the mixture from chromatographic technique will enter into the spectroscopic technique through an interphase. The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. LC-NMR is the hyphenated technique in which HPLC is combined with the NMR. There are various NMR probes that can be used for increasing the efficiency of LC-NMR. This technique is widely used for the analysis of complex mixtures which contain unknown impurities, natural products and synthetic polymers. In LC-NMR LC does the separation and NMR does the identification of separated components. Further hyphenated technique such as LCNMR/MS are also described.

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