Murine Insulinoma Cell-Conditioned Medium with ΒETA2/Neurod1 Transduction Efficiently Induces the Differentiation of Adipose-Derived Mesenchymal Stem Cells into β-Like Cells both In Vitro and In Vivo

Hidetoshi Eguchi¹, Hitoshi Okochi^2*, Hiroaki Nagano^1*, Tatsuo S Hamazaki², Masaki Mori¹, Yuichiro Doki¹, Takashi Miki⁶, Eri Mukai⁶, Eun Young Lee⁶, Toshinori Ito⁵, Masahiro Tanemura⁴, Koichi Kawamoto^1#, Shogo Kobayashi¹, Hiroshi Wada¹, Naoki Hama¹, Yoshito Tomimaru¹, Satsuki Fukuda², Jun Koseki³, Naohiro Nishida³, Hideshi Ishii³, Masamitsu Konno^3# and Shigeharu Yabe^2#

¹Department of Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan, E-mail: hokochi@ri.ncgm.go.jp

²Department of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan, E-mail: hokochi@ri.ncgm.go.jp

³Department of Frontier Science for Cancer and Chemotherapy, National Hospital Organization Kure Medical Center, Hiroshima, 737-0023, Japan, E-mail: hokochi@ri.ncgm.go.jp

⁴Department of Surgery and Institute for Clinical Research, National Hospital Organization Kure Medical Center, Hiroshima, 737-0023, Japan, E-mail: hokochi@ri.ncgm.go.jp

⁵Department of Complementary and Alternative Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan, E-mail: hokochi@ri.ncgm.go.jp

⁶Department of Medical Physiology, Chiba University, Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan, E-mail: hokochi@ri.ncgm.go.jp

^#Contributed equally to this work

^*Corresponding Author(s):