Zhi-li Suo
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing,
China
Research Article
A New Nuclear DNA Marker Revealing Both Microsatellite Variations and Single Nucleotide Polymorphic Loci: A Case Study on Classification of Cultivars in Lagerstroemia indica L
Author(s): Zhi-li Suo, Wen-ying Li, Xiao-bai Jin and Hui-jin ZhangZhi-li Suo, Wen-ying Li, Xiao-bai Jin and Hui-jin Zhang
Plant cultivars are important germplasm resources for socio-economic development. However, it is difficult to conduct accurate genetic evaluation on plant diversity solely on the basis of morphological traits which are easily affected by environmental conditions and may change during developmental stages of the plant. Developing DNA markers with high resolution and sensitivity, especially at cultivar level, is a global challenge. We report a good methodology for rapid and efficient detection of plant genetic diversity at cultivar level. A unique nucleotide molecular formula (NMF) was constructed for each crape myrtle using polymorphic nucleotide sites. Our results showed that the DNA sequence from chromatin remodeling gene region of the ubiquitin-proteasome system is useful for molecularly characterizing crape myrtle cultivars. This DNA marker technique will be of significant value fo.. View More»
DOI:
10.4172/1948-5948.1000296