Zhang Yong-Pan
China
Research Article
Analysis of Escherichia Coli Inducing Salpingitis-Peritonitis in Layer Geese by
Two-Dimensional Electrophoresis and MALDI-TOF/TOF
Author(s): Jin Wen-Jie, Zhang Yong-Pan, Qian Wen-Zheng, Zhang Di, Shao Hong-Xia, Qian Kun and Qin Ai-Jian
Jin Wen-Jie, Zhang Yong-Pan, Qian Wen-Zheng, Zhang Di, Shao Hong-Xia, Qian Kun and Qin Ai-Jian
Geese salpingitis-peritonitis mainly happens in layer geese during egg peak period and leads serious economic losses. This study was conducted in different resource E. coli inducing Geese salpingitis-peritonitis and Geese or chicken fibrinous pericarditis-perihepatitis-airsacculitis respectively. The molecular mechanism underlying pathogenicity of Geese salpingitis-peritonitis E. coli was investigated through analysis of the special protein of this E. coli using proteomics and bioinformatics techniques. Two-dimensional electrophoresis (2-DE) analysis showed a total of 42 protein spots only expressed in Geese salpingitis-peritonitis E. coli. A total of 21 proteins were identified by MALDI TOF/TOF mass spectral analysis. According to gene ontology (GO) and Pathway analysis, special expressed proteins in Geese salpingitis-peritonitis E. coli were mainly involved in the synthesis of macro.. View More»
DOI:
10.4172/2155-9597.1000198