Journal of Nanomedicine & Nanotechnology

Yuan-Cheng Cao

Publications

• Research Article
  An Organic Nanoparticle based High Signal Amplification Immunoassay with Improvement of Nonspecific Binding
  Author(s): Zhan Wang and Yuan-Cheng CaoZhan Wang and Yuan-Cheng Cao
  
  A high signal amplification immunoassay method based on Fluorescein Diacetate (FDA) organic nanoparticle was proposed here. FDA nanoparticles were prepared in a ball mill and centrifugation was applied to get the size-uniformed SDS stabilized particle (FDA@SDS). Further SEM images confirmed the particle size was well dispersed in solution and particle size analysis results showed that the particle size 90% was distributed in the range of 200 nm to 500 nm. Mass Spectrometry results showed that the antibody can directly attach to the FDA@SDS particles and standard sandwich immunoassay confirmed the bioactivity of the antibody on the FDA@SDS particle surface (FDA@SDS-IgG). And then Zeta potential test results showed that the blocking reagents BSA and Casein can successfully block the unsatisfied sites on the FDA@SDS-IgG surface, which resulted in the improvement of nonspecific binding an.. View More»
  DOI: 10.4172/2157-7439.1000207

  Abstract PDF