Yongyut Prompunjai
Thailand
Research Article
Alternative Malaria Diagnostic Tools: Evaluation of Plasmodium falciparum Detection along Thailand?s Border by Loop-Mediated Isothermal Amplification (LAMP) and Immunochromatographic Test (ICT)
Author(s): Salakchit Chutipongvivate*, Yongyut Prompunjai, Wanvisa Neadruengsang and Supaporn WangsiricharoenSalakchit Chutipongvivate*, Yongyut Prompunjai, Wanvisa Neadruengsang and Supaporn Wangsiricharoen
Introduction: Thailand has a national goal to eliminate malaria from 80 percent of the country by 2020. However, malaria-endemic areas still exist along the forested Thailand’s borders. An accurate and prompt diagnosis is the key to effective disease management. This aim of this study was to evaluate the application of LAMP as an alternative tool for falciparum malaria diagnosis of clinical suspicion of plasmodium infection in the border area, compared to those of ICT and microscopy using nested PCR as the reference detection method.
Materials and Methods: One hundred and four of blood samples were obtained from patients who were diagnosed malaria positive by clinical diagnosis or subjected to suffering from malaria as observed from clinical history. The primer set used for LAMP was designed on the basis of nucleotide sequence of 18S rRN.. View More»